Online Dilution Calculator

The dilution calculator equation

The tech-publish dilution calculator is based on the following equation:

Concentration_(Initial) x Volume_(Initial) = Concentration_(final) x Volume_(final)

The standard abbreviation for this equation is: C₁V₁ = C₂V₂

An example of a dilution calculation using the dilution calculator

What volume of a given 20 mM stock solution is required to make 40ml of a 100 μM solution?

Using the equation C₁V₁ = C₂V₂, where C₁=20 mM, C₂=100 μM, V₂=40 ml and V₁ is the unknown:

1. Enter 20 into the Concentration (Initial) box and select the correct unit (millimolar)
2. Enter 100 into the Concentration (final) box and select the correct unit (micromolar)
3. Enter 400 into the Volume (final) box and select the correct unit (milliliter)
4. Press calculate
5. The answer of 200 microliter (0.2 ml) appears in the Volume (Initial) box