Online Dilution Calculator
Determine the amount of dilution needed to make a stock solution.
The dilution calculator equation
The tech-publish dilution calculator is based on the following equation:
Concentration(Initial) x Volume(Initial) = Concentration(final) x Volume(final)
The standard abbreviation for this equation is: C1V1 = C2V2
An example of a dilution calculation using the dilution calculator
What volume of a given 20 mM stock solution is required to make 40ml of a 100 μM solution?
Using the equation C1V1 = C2V2, where C1=20 mM, C2=100 μM, V2=40 ml and V1 is the unknown:
- Enter 20 into the Concentration (Initial) box and select the correct unit (millimolar)
- Enter 100 into the Concentration (final) box and select the correct unit (micromolar)
- Enter 400 into the Volume (final) box and select the correct unit (milliliter)
- Press calculate
- The answer of 200 microliter (0.2 ml) appears in the Volume (Initial) box