Preparation and standardization of 0.1 N Sulfuric acid USP Volumetric Solution

1.  PREPARATION :

Add slowly, with stirring, 2.7 mL of Sulfuric acid to a sufficient quantity of water to make 1000 mL.

2. STANDARDISATION :

Accurately weigh about 0.50 g of Tromethamine, previously dried at 105°C for 3 hours. Dissolve in 50 mL of water, and add 2 drops of Bromocresol green TS. Titrate with 0.1 N Sulfuric acid to a pale yellow end point.

Each 121.14 mg of Tromethamine is equivalent to 1 mL of 1 N Sulfuric acid.

Calculation :

Normality (N) = [Weight of Tromethamine (in g)]/[ 0.12114 x Titration Reading (in mL)]

Limit : 0.1 N ± 5 % (0.0950 N – 0.1050 N)

3. PREPARATION OF INDICATORS / REAGENTS :

a. Bromocresol green TS:

Dissolve 50 mg of Bromocresol green in 100 mL of alcohol and filter if necessary.

4. STORAGE CONDITION :

Store in glass-stoppered bottles.