Instrument Calibration Protocol of Polarimeter

Instrument	Polarimeter
Instrument ID. No.
Ref. SOP No.
Model No.	P-2000
Calibration frequency	Quarterly – 5 days
Date of calibration
Next due date

1. Angle of rotation of Sucrose solution :

Weigh and transfer accurately 10 g of previously dried Sucrose AR to a 100 ml volumetric flask, dissolve and dilute with water and mix (10 %).

Make the different concentrations of Sucrose solution 20%, 30%, 40% and 50% in the same manner like 10 %. 

Balance ID: __________________.

Sucrose AR, Make: _________________, Valid up to :________________.

10 % w/v Sucrose AR Solution: _______________ g Sucrose AR  –> 100 with water

20 % w/v Sucrose AR Solution: _______________ g Sucrose AR  –>100 with water

30 % w/v Sucrose AR Solution: _______________ g Sucrose AR  –>100 with water

40 % w/v Sucrose AR Solution: _______________ g Sucrose AR  –> 100 with water

50 % w/v Sucrose AR Solution: _______________ g Sucrose AR  –> 100 with water

Temperature: 25 ± 0.5 °C

Angle of Rotation

Concentration	Obs.1	Obs.2	Obs.3	Obs.4	Obs.5	Average	Limits
10 %							6.643 – 6.687
20 %							13.261 – 13.349
30 %							19.864 – 19.996
40 %							26.442 – 26.618
50 %							33.004 – 33.226

Remarks: Calibration Satisfactory/ Not Satisfactory

	Prepared By	Checked By	Approved By
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