Instrument Calibration Protocol of Spectrofluorometer

INSTRUMENT	Spectrofluorometer
Instrument Code No.
Reference SOP No.
Model No.	FP-8300
Make	JASCO
Date of Calibration
Next Due Date
Frequency	Quarterly -5 days

1. Wavelength Accuracy: For Excitation Monochromator :

Sr. No.	Measured	Mean Difference	Criteria	Result
1			253.7 ± 1.5 nm (252.2 nm to 255.2 nm)	Pass □              Fail □
2			—	—
3			—	—
1			365.0 ± 1.5 nm (363.5 nm to 366.5 nm)	Pass □              Fail □
2			—	—
3			—	—
1			435.8 ± 1.5 nm (434.3 nm to 437.3 nm)	Pass □              Fail □
2			—	—
3			—	—
1			546.1 ± 1.5 nm (544.6 nm to 547.6 nm)	Pass □              Fail □
2			—	—
3			—	—

Done By :                                                                   Checked By :

For Emission Monochromator:

Sr. No.	Measured	Mean Difference	Criteria	Result
1			253.7 ± 1.5 nm (252.2 nm to 255.2 nm)	Pass □              Fail □
2			—	—
3			—	—
1			365.0 ± 1.5 nm (363.5 nm to 366.5 nm)	Pass □              Fail □
2			—	—
3			—	—
1			435.8 ± 1.5 nm (434.3 nm to 437.3 nm)	Pass □              Fail □
2			—	—
3			—	—
1			546.1 ± 1.5 nm (544.6 nm to 547.6 nm)	Pass □              Fail □
2			—	—
3			—	—

Done By :                                                                   Checked By :

2. Wavelength Repeatability:

For Excitation Monochromator:

Sr. No.	Measured	Mean Difference	Criteria	Result
1			546.1 ± 1.0 nm (545.1 nm to 547.1 nm)	Pass □              Fail
2			—	—
3			—	—

Done By :                                                                   Checked By :

For Emission Monochromator :

Sr. No.	Measured	Mean Difference	Criteria	Result
1			546.1 ± 1.0 nm (545.1 nm to 547.1 nm)	Pass □              Fail □
2			—	—
3			—	—

Done By :                                                                   Checked By :

3. Sensitivity:

Peak height of Water Raman Peak (Height of Peak – Height of Baseline) =

      Noise Level (Maximum noise level – Minimum noise level) =

Ratio = Peak height of Water Raman Peak/ Noise Level

Ratio =

Signal to noise ratio

Peak height of Water Raman Peak	Noise Level	Ratio	Criteria	Result
			NLT 170	Pass □          Fail □

Done By :                                                                Checked By :

  Remarks: The instrument is calibrated & qualified / Out of calibration & not qualified for use.

—	Calibrated By	Checked By	Approved By
Signature
Date
Name
Department	Quality Control	Quality Control	Quality Control