October 13, 2021: Pharmaceutical Interview Questions and Answers #DirectInoculationOfTheCultureMediumForSterilityTesting 1. Explain the direct inoculation of the culture medium for sterility testing? ● Transfer the quantity of the preparation to be examined prescribed in following Table directly into the culture medium so that the volume of the product is not more than 10% of the volume …
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October 12, 2021: Pharmaceutical Interview Questions and Answers #FiltersForSterilityTesting #MembraneFiltrationMethodForSterilityTesting #SterilityTestingForAqueousSolutions #SterilityTestingForSolubleSolids #SterilityTestingForOilsAndOilySolutions #SterilityTestingForOintmentsAndCreams 1. Types of filter preferable for sterility testing using filtration method. Cellulose nitrate filters: For aqueous, oily and weakly alcoholic solutions Cellulose acetate filters: Strongly alcoholic solutions 2. What is preferred filter diameter for sterility testing using filtration method? 50 mm …
October 08, 2021: Pharmaceutical Interview Questions and Answers #CompositionOfFluidThioglycollateMedium? #CompositionOfSoyaBeanCaseinDigestMedium #SterilityTestingSampleQuantityAndDiluent 1. What is the composition of Fluid thioglycollate medium? The composition of Fluid thioglycollate medium is as follows: Name of ingredient Weight L-Cystine 0.5 g Agar 0.75 g Sodium chloride 2.5 g Glucose monohydrate/anhydrous 5.5/5.0 g Yeast extract (water-soluble) 5.0 g Pancreatic digest of …
October 07, 2021: Pharmaceutical Interview Questions and Answers #SterilityTestValidation #EnvironmentForSterilityTesting #SterilityTestingSample 1. How to interpret sterility test validation outcome? There are two outcomes with the sterility test: (1) Clearly visible growth – test sample and the control tubes are equivalent (2) No clearly visible comparable growth – Test needs to be modified and the validation …
October 06, 2021: Pharmaceutical Interview Questions and Answers #SterilityTestGrowthMediumGPT #IncubationConditionsAndTimesSterilityTesting 1. What types of growth promotion test (GPT) to be done for growth medium used to perform sterility testing? ● GPT for Fluid thioglycollate medium shall be done using Clostridium sporogenes, P. aeruginosa, S. aureus; ● GPT for Soya-bean casein digest medium shall be done …
October 05, 2021: Pharmaceutical Interview Questions and Answers #SterilityTest #SterilityTestingMethods #MembraneFiltrationMethodComponents #GrowthMediumsForSterilityTesting 1. Is sterility test qualitative or quantitative test? The sterility test is a qualitative test. Results are written as presence or absence of turbidity based on growth for bacteria and fungi in media. 2. Which are commonly used sterility testing methods? ● Membrane …
October 04, 2021: Pharmaceutical Interview Questions and Answers #MicrobialConcentrationByOpticalDensity #CommonSourcesOfPyrogens #MethodUsedToTestPyrogen 1. Explain the method of measurement of microbial concentration in suspension by optical density? A spectrophotometer method is used to measures turbidity of inoculum suspension. When light passes through a suspension of microorganisms, light gets scattered and the amount of scatter is an indication …
October 03, 2021: Pharmaceutical Interview Questions and Answers #SpecifiedOrganismsAndSource #InterpretSpecificationLimitForMicrobiologyTest 1. When test for specified organisms found positive, what it indicates for each organism? Name of specified organism Source Risk Escherichia coli Gut flora The organism indicates fecal contamination Salmonellae Gut flora Fecal contamination with high pathogenicity S. aureus Human skin human skin and …
October 02, 2021: Pharmaceutical Interview Questions and Answers #ApprovedMicrobialCultureSource #SamplePreparationForBioburdenTest 1. Which are the various organization from where approved culture collections of different train of microorganisms can be obtained? ● American Type Culture Collection (ATCC) ● National Collection of Industrial and Marine Bacteria (NCIMB) ● Collection of Institute Pasteur (CIP) ● Imperial Mycological Institute (IMI) …
October 01, 2021: Pharmaceutical Interview Questions and Answers #TotalViableAerobicCount #BioburdenTestMethodValidation 1. What is Total viable aerobic count? Total viable aerobic count is designed to count the number of microorganisms (as colony forming units, CFUs) in a non-sterile pharmaceutical product or raw materials. It is father divided into two parts, total aerobic microbial count (TAMC) and …
