Pharma-Interview

September 30, 2021: Pharmaceutical Interview Questions and Answers #LactophenolCottonBlueStain #FungalStaining #ZiehlNeelsenStain #AcidFastStain 1. What is Lactophenol cotton blue stain or fungal staining? Lactophenol cotton blue stain is used to examining yeast and filamentous fungi under microscope. Phenol acts as a disinfectant to kill living organisms Lactic acid used to preserve the fungal structures Cotton blue …

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September 29, 2021: Pharmaceutical Interview Questions and Answers #Endospore staining #SchaefferFultonStaining 1. Which indicators are used for spore staining? Malachite green (a triarylmethane dye) and a safranin (an azonium compound) counterstain is useful tool in identifying the presence or absence of spores. This is referred to as the Schaeffer-Fulton stain. 2. Explain the Schaeffer-Fulton staining …

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September 28, 2021: Pharmaceutical Interview Questions and Answers #AllAboutGram’sStaining #Gram’sStainingPrinciple #Gram’sStainingSteps #Gram’sStainingReagents 1. What is Gram-stain? The Gram stain is an important technique for identification of bacteria. It divides bacteria into two groups, Gram-positives and Gram-negatives. 2. How the Gram-staining technique is useful? Gram-staining technique that allows to visualize the morphological types of bacteria using …

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September 27, 2021: Pharmaceutical Interview Questions and Answers #ObjectiveOfMicrobialIdentification #TaxonomicTermsMicrobialIdentification #FirstStepofMicrobialIdentification #ColonyAndCellularMorphology 1. What is the objective of microbial identification? The objective of microbial identification is to differentiate one microbial isolate from another with respect to family (genus) and a species or a particular strain. 2. What are the taxonomic terms for microbial identification? ● …

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September 26, 2021: Pharmaceutical Interview Questions and Answers #TypesOfMicrobialIdentificationMethods #DifferencesBetweenPhenotypeAndGenotype #DifferentStainingTechniques 1. Explain types of microbial identification methods. Identification methods can be divided into two types: ● Phenotypic ● Genotypic 2. What are the differences between Phenotype and Genotype? ● “Genotype” is an organism’s full hereditary information. ● “Phenotype” is an organism’s actual observed properties, …

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September 24, 2021: Pharmaceutical Interview Questions and Answers #SampleNeutralization #WhatIsPyrogensAndEndotoxins #SourceOfPyrogensAndEndotoxins 1. What should be done during microbiology testing if sample exhibit antimicrobial activity? When the sample possesses antimicrobial activity, it that requires neutralization. Following are commonly used principle for neutralization: ● Chemical neutralization ● Enzymatic neutralization ● Dilution 2. What is Pyrogens and endotoxins? …

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September 23, 2021: Pharmaceutical Interview Questions and Answers #PureCulture # MostCommonMethodToIsolateIndividualCells #StreakPlateMethod 1. What is pure culture? A pure culture is a population of cells or multicellular organisms growing in the absence of other species or types in which cells are genetic clones of one another. 2. What is most common method to isolate individual …

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September 22, 2021: Pharmaceutical Interview Questions and Answers #GoodPracticeInMicrobiologicalLaboratory #AsepticTechniqueInMicrobiologicalLaboratory #Asepsis 1. Give few examples of good practice in a microbiological laboratory. ● Required equipment and media should sterilized prior to use.● Sterilized equipment and media should be maintained and stored in such a way that it should not be contaminated.● Frequently disinfect hands and …

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September 21, 2021: Pharmaceutical Interview Questions and Answers #AcceptanceCriteriaOfMicrobiologicalQualityNonSterileDosageForm #RisksBecauseOfMicroorganism 1. What are the acceptance criteria for microbiological quality of non-sterile dosage forms and raw materials? Application TAMC TYMC Specified microorganisms Non-aqueous preparations for oral use 1000 100 Escherichia coli absent in 1 g or 1 mL Aqueous preparations for oral use 100 10 Escherichia …

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September 20, 2021: Pharmaceutical Interview Questions and Answers #StepsForManufacturingOfCultureMedia 1. What are the steps involved in the manufacturing of culture media? a. Collection of prerequisite ● Decide batch size● Vessel for holding or dispensing the media● Weighing balance● Petri dishes/ Containers● Dehydrated media b. Rehydration of media ● Media powder is rehydrated by mixing the …

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