Instrument Calibration Protocol for FT-IR (Fourier-transform infrared spectroscopy)

Instrument	:	FT-IR
Instrument Code Number	:
Refer Operating SOP No.	:
Model No.	:	Spectrum Two
Date of Calibration	:
Make	:	Perkin Elmer
Next Due Date	:

1. Perform auto adjustment:

Observation: Auto adjustment found satisfactory / not satisfactory.

2. Verification of Wave number Scale :

Sr. No.	Peak to be observe at Wave numbers	Measured Wave number	Error	Limits in wave numbers (cm-1)	Remark
1.	3060.0 cm-1			3059.0 – 3061.0 cm-1
2.	2849.5 cm-1			2848.5 – 2850.5 cm-1
3.	1942.9 cm-1			1941.9 – 1943.9 cm-1
4.	1601.2 cm-1			1600.2 – 1602.2 cm-1
5.	1583.0 cm-1			1582.0 – 1584.0 cm-1
6.	1154.5 cm-1			1153.5 – 1155.5 cm-1
7.	1028.3 cm-1			1027.3 – 1029.3 cm-1

3. Resolution performance of Instrument :

Sr. No.	% Transmittance difference between	Observed Results	Limits	Remarks
1.	2849.5 cm-1   & 2870 cm-1		NLT 0.33
2.	1583 cm-1 & 1589 cm-1		NLT 0.08

Remarks: Calibration satisfactory / Not satisfactory

Calibrated By

Signature/Date                       

Checked By

Signature/Date                       

Approved By

Signature/Date