Ion Chromatography Calibration Protocol

Instrument : Ion Chromatography

Instrument Code Number :

Refer Operating SOP No. :

Model No     :

Make:

Date of Calibration  :

Next due date:

1. Detector Linearity:

    Nitrate Standard Solution 1000mg/lNO₃

    Batch / Lot No. : ________________                Expiration date: ___________

    Observation Table:

Sr. No.	Concentration (ppm)	Height  (µS)

Concentration Vs Height
Observed Correlation coefficient
Limit	≥ 0.999	Should be Linear.

2. Noise Drift:

    Sample: Inject 25 µL of Milli Q Water once.

    Observation Table:

    Data for Detector Noise 

Segment No.	Noise, nS
Average, nS
Limit, nS	2.0
Result

Data for Detector Drift           

20 minute, Drift, nS	Drift, nS/hr	Limit, nS/hr	Result
		20

3. Injector Precision:

 Note: Run the single injection of DI (De Ionized) water with full loop capacity before standard injection.

Nitrate Standard Solution 1000mg/lNO₃

Batch / Lot No.: ________________,              

Expiration date: ____________

Observation Table:

Name	Area (µS * min)
Average
Std dev.
% RSD
Limit	1.0 %
Result

4. Injector Linearity:

Nitrate Standard Solution 1000mg/lNO₃

  Batch / Lot No. : ________________,               Expiration Date: ____________

Observation Table:   

Sr. No.	Injection volume (µl)	Area (µS * min)

Injection volume Vs Area
Correlation coefficient
Limit	≥ 0.999	Should be Linear.

5. Injector Carry Over Check:

Observation Table:

Name	Retention time (min)	Area (µS * min)
Carry over	–
Limit	–	0.1 %
Result	–

6. Gradient Pump Accuracy Test:

Inject 25 µl of Milli Q water.

Observation Table:

Step Accuracy – Valves A, B

Theoretical value (%)	Conductivity (µS)	Calculated value (%)	Deviation (%)	Limit (±, %)	Result
				N/A	–
				2.0
				2.0
				2.0
				2.0

Step Accuracy – Valves C, D

Theoretical value (%)	Conductivity (µS)	Calculated value (%)	Deviation (%)	Limit (±, %)	Result
				N/A	–
				2.0
				2.0
				2.0
				2.0

Step Noise – Valves A, B    

Step (%)	Noise (µS)	Calculated Noise (%FS)	Limit ( %)	Result
			NMT 0.20%
			NMT 0.20 %
			NMT 0.20 %
			NMT 0.20 %
			NMT 0.20 %

Step Noise – Valves C, D

Step (%)	Noise (µS)	Calculated Noise (%FS)	Limit ( %)	Result
			NMT 0.20 %
			NMT 0.20 %
			NMT 0.20 %
			NMT 0.20 %
			NMT 0.20 %

7. Flow rate Accuracy and Precision Test:

    Balance ID: _________________

   Weight of 25 ml measuring cylinder: _________________ g

    Temperature: _________________

    Observation Table:  

Segment	Weight of measured eluent in measuring cylinder in ‘g’	Calculated eluent i.e. Calculated Flow rate in ml/min.	Deviation from 1.00 ml/min. (Limit: NMT ± 0.050)
Average Flow
Std Dev.
% RSD (Limit: NMT 2.0 %)

REMARKS: Calibration satisfactory /Not satisfactory

Prepared By

Signature/ Date

Checked By

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Approved By

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