Preparation and standardization of 0.02 M Zinc Sulfate USP Volumetric Solution
1. PREPARATION:
Dissolve 5.76 g of Zinc sulfate in water to make 1000 mL.
2. STANDARDISATION:
Accurately measure about 10 mL of 0.02 M Disodium edetate VS into a 125 mL conical flask, and add, in the order given, 10 mL of Acetic acid-ammonium acetate buffer TS, 50 mL of Alcohol, and 2 mL of Dithizone TS. Titrate with the 0.02 M Zinc sulfate solution to a clear, rose-pink color.
Calculation:
Molarity (M) = [mL of Edetate x M Edetate disodium]/[mL of ZnSO4]
Limit: 0.02 M ± 5 % (0.019 M – 0.021 M)
3. STORAGE CONDITION:
Store in glass stoppered bottles, protected from light.