Instrument Calibration Protocol for Atomic Absorption Spectrometer (AAS)

Instrument	Atomic Absorption Spectrometer
Instrument ID. No.
Ref. SOP No.
Model No.
Calibration frequency	Half Yearly (-15 days).
Date of calibration
Next due date

Note:

• Prior to start calibration activity, required glassware shall be rinsed with the respective acid diluents as per standard bottle label. For example, use 1% HNO3 in case of calibration performing with Manganese & Nickel use 1% HCl in case of calibration performing with Chromium standard.
•   The blank shall be prepared as similar to the rinse volumetric flask with the acid diluents. For 

  Example, use 1% HNO3 in case of calibration performing with Manganese & Nickel, and use

  1% HCl in case of calibration performing with Chromium standard.

• The graphite furnace auto sampler wash vessel must be filled with suitable wash diluents. For example: Use 1% HNO3 for Manganese standard solution as a wash diluent.
• Prior to calibration, graphite shall be checked visually and if any carbon deposition observed it shall be replaced with the new one.

1. Air-Acetylene flame test using Nickel Standard:

     Nickel Standard Batch Number:

           Validity:

           Preparation of 1% HNO3:

Preparation of Nickel Standard (10 ppm):

Pipetted out _______ (1.0 mL) of 1000 mg/L Nickel standard solution in 100 mL volumetric    

flask and diluted it up to the mark with water, Shaked well.

Sr. No.	Name of Standard	No. of Resamples	Limit	Result	% RSD (NMT 1.0%)
1	Nickel (10 ppm)	10	Absorbance (NLT 0.50)

Remark: The Instrument complies / does not comply Air-Acetylene flame test by using Nickel Standard.

  Analyzed By:                                                                        Checked By:

  Date:                                                                                      Date:

2. Nitrous Oxide-Acetylene flame test using Chromium standard:

Chromium Standard Batch Number:

Validity:

Preparation of 1% HCl:

Preparation of Chromium Standard (3ppm):

1) Pipetted out _______ (1.0 mL) of 1000 mg/L Chromium standard solution in 10 mL volumetric flask and diluted it upto the mark with water, shaked well.

2) Further pipetted out _______ (3.0 mL) of above solution in 100 mL volumetric flask and diluted it upto the mark with water, shaked well.

Sr. No	Name of Standard	No. of Resamples	Limit	Result	% RSD (NMT 1.5%)
1	Chromium (3 ppm)	10	Absorbance (NLT 0.16)

Remark: The Instrument complies / does not comply Nitrous Oxide-Acetylene flame test using Chromium standard.

  Analyzed By:                                                                                   Checked By:

  Date:                                                                                                Date:

3. Furnace test using Manganese standard:

Note: Graphite visually checked is found Ok / Not Ok

Manganese Standard Batch Number:                        

Validity:        

Preparation of 1% HNO3:

Preparation of Manganese Standard (20ppb):

1) Pipetted out ________ (1.0 mL) of 1000 mg/L Manganese standard in 10 mL volumetric flask and diluted it upto the mark with water, shaked well.

2) further, pipetted out _______ (1.0 mL) of above solution in 100 mL volumetric flask and diluted it upto the mark with water, shaked well.

3)  further, pipetted out _______ (2.0 mL) of above solution in 100 mL volumetric flask and diluted it upto the mark with water, shaked well.

Sr. No	Name of Standard	No. of Resamples	Limit	Result	% RSD (NMT 5.0%)
1	Manganese (20 ppb)	5	Absorbance (NLT 0.80)

Remark: The Instrument complies / does not comply furnace test using Manganese standard.

Analyzed By:                                                                      Checked By:

Date:                                                                                  Date:

Conclusion: Calibration found satisfactory/not satisfactory.

Calibrated By (Sign/ Date)

Checked By (Sign/ Date)