Pharmaceutical Interview Questions and Answers

October 08, 2021: Pharmaceutical Interview Questions and Answers

#CompositionOfFluidThioglycollateMedium? #CompositionOfSoyaBeanCaseinDigestMedium #SterilityTestingSampleQuantityAndDiluent

1. What is the composition of Fluid thioglycollate medium?

The composition of Fluid thioglycollate medium is as follows:

Name of ingredientWeight
L-Cystine0.5 g
Agar0.75 g
Sodium chloride2.5 g
Glucose monohydrate/anhydrous5.5/5.0 g
Yeast extract (water-soluble)5.0 g
Pancreatic digest of casein15.0 g
Sodium thioglycollate or0.5 g
Thioglycollic acid0.3 ml
Resazurin sodium solution (1 g/l of resazurin sodium), freshly prepared1.0 ml
Water R1000 ml

pH after sterilization 6.9 to 7.3.

2. What is the composition of Soya-bean casein digest medium?

The composition of Soya-bean casein digest medium is as follows:

Name of ingredientWeight
Pancreatic digest of casein17.0 g
Papaic digest of soya-bean meal3.0 g
Sodium chloride5.0 g
Dipotassium hydrogen phosphate2.5 g
Glucose monohydrate/anhydrous2.5/2.3 g
Water R1 000 ml

pH after sterilization 7.1 to 7.5.

3. While sterility testing using membrane filtration, what should be suitable volume of diluent and sample quantity of product to be examined prescribed?

Liquids sample

Minimum quantity to be used for each medium Quantity per containerMinimum quantity to be used for each medium unless otherwise justified and authorized
• less than 1 mlThe whole contents of each container
• 1-40 mlHalf the contents of each container but not less than 1 ml
• greater than 40 ml and not greater than 100 ml20 ml
• greater than 100 ml10 per cent of the contents of the container but not less than 20 ml
Antibiotic liquids1 ml

Insoluble preparations, creams and ointments sample

Minimum quantity to be used for each medium Quantity per containerMinimum quantity to be used for each medium unless otherwise justified and authorized
Insoluble preparations, creams and ointments to be suspended or emulsifiedUse the contents of each container to provide not less than 200 mg

Solids sample

Minimum quantity to be used for each medium Quantity per containerMinimum quantity to be used for each medium unless otherwise justified and authorized
• less than 50 mgThe whole contents of each container
• 50 mg or more but less than 300 mgHalf the contents of each container but not less than 50 mg
• 300 mg – 5 g150 mg
• greater than 5 g500 mg

Reference: WHO, Document QAS/11.413 FINAL, March 2012

Know more interview Questions and Answers published previously go to page: https://tech-publish.com/pharmaceutical-interview-questions-and-answers.

Scroll to Top