Preparation and Standardisation of 0.01 M Edetate Disodium USP Volumetric Solution
1. PREPARATION :
Dissolve 3.7224 g of Disodium Edetate in water to make 1000 mL.
2. STANDARDISATION :
Accurately weigh about 100 mg of Chelometric standard Calcium carbonate, previously dried at 110°C for 2 hours and cooled in a desiccator, transfer to a 200 mL beaker, add 5 mL of water, and swirl to form a slurry. Cover the beaker with a watch glass, and introduce 1 mL of diluted Hydrochloric acid from a pipette inserted between the lip of the beaker and the edge of the watch glass. Swirl the contents of the beaker to dissolve the Calcium carbonate. Wash down the sides of the beaker, the outer surface of the pipette, and the watch glass with water, and dilute with water to about 50 mL. While stirring the solution, preferably with a magnetic stirrer, add about 80 mL of the 0.01 M Disodium Edetate solution from a 50 mL burette. Add 10 mL of Sodium hydroxide TS and 150 mg of Hydroxy napthol blue and continue the titration with 0.01 M Disodium Edetate solution to a blue endpoint.
Molarity (M) = (Weight of Calcium carbonate (in g) x 1000)/ 100.09 x Volume of 0.01 M Disodium Edetate consumed (in mL)
Limit : 0.01 M ± 5 % (0.0095 M – 0.0105 M)
3. PREPARATION OF INDICATORS / REAGENTS :
a. Diluted Hydrochloric acid :
Dilute 22.6 mL Hydrochloric acid to 100 mL with water.
b. Sodium hydroxide TS :
Dissolve 4.0 g of Sodium hydroxide in water to make 100 mL.
4. STORAGE CONDITION :
Store in glass stoppered bottles.