Pharmaceutical Interview Questions and Answers

October 05, 2021: Pharmaceutical Interview Questions and Answers

#SterilityTest #SterilityTestingMethods #MembraneFiltrationMethodComponents #GrowthMediumsForSterilityTesting

1. Is sterility test qualitative or quantitative test?

The sterility test is a qualitative test. Results are written as presence or absence of turbidity based on growth for bacteria and fungi in media.

2. Which are commonly used sterility testing methods?

● Membrane filtration: This is further classified as open method and closed system method.

● Direct inoculation method

3. What components required performing sterility testing by membrane filtration method?

● Filter with pore size 0.45 μm

● Filter diameter about 50 mm

● Cellulose nitrate filters for aqueous, oily, and weak alcoholic solutions

● Cellulose acetate filters for strong alcoholic solutions

4. What are the growth mediums used to perform sterility testing?

● Fluid Thioglycollate Medium for anaerobic bacteria and it also isolates aerobic bacteria

● Soya-bean Casein Digest Medium for the isolation of fungi and aerobic bacteria

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