October 08, 2021: Pharmaceutical Interview Questions and Answers
#CompositionOfFluidThioglycollateMedium? #CompositionOfSoyaBeanCaseinDigestMedium #SterilityTestingSampleQuantityAndDiluent
1. What is the composition of Fluid thioglycollate medium?
The composition of Fluid thioglycollate medium is as follows:
| Name of ingredient | Weight |
| L-Cystine | 0.5 g |
| Agar | 0.75 g |
| Sodium chloride | 2.5 g |
| Glucose monohydrate/anhydrous | 5.5/5.0 g |
| Yeast extract (water-soluble) | 5.0 g |
| Pancreatic digest of casein | 15.0 g |
| Sodium thioglycollate or | 0.5 g |
| Thioglycollic acid | 0.3 ml |
| Resazurin sodium solution (1 g/l of resazurin sodium), freshly prepared | 1.0 ml |
| Water R | 1000 ml |
pH after sterilization 6.9 to 7.3.
2. What is the composition of Soya-bean casein digest medium?
The composition of Soya-bean casein digest medium is as follows:
| Name of ingredient | Weight |
| Pancreatic digest of casein | 17.0 g |
| Papaic digest of soya-bean meal | 3.0 g |
| Sodium chloride | 5.0 g |
| Dipotassium hydrogen phosphate | 2.5 g |
| Glucose monohydrate/anhydrous | 2.5/2.3 g |
| Water R | 1 000 ml |
pH after sterilization 7.1 to 7.5.
3. While sterility testing using membrane filtration, what should be suitable volume of diluent and sample quantity of product to be examined prescribed?
Liquids sample
| Minimum quantity to be used for each medium Quantity per container | Minimum quantity to be used for each medium unless otherwise justified and authorized |
| • less than 1 ml | The whole contents of each container |
| • 1-40 ml | Half the contents of each container but not less than 1 ml |
| • greater than 40 ml and not greater than 100 ml | 20 ml |
| • greater than 100 ml | 10 per cent of the contents of the container but not less than 20 ml |
| Antibiotic liquids | 1 ml |
Insoluble preparations, creams and ointments sample
| Minimum quantity to be used for each medium Quantity per container | Minimum quantity to be used for each medium unless otherwise justified and authorized |
| Insoluble preparations, creams and ointments to be suspended or emulsified | Use the contents of each container to provide not less than 200 mg |
Solids sample
| Minimum quantity to be used for each medium Quantity per container | Minimum quantity to be used for each medium unless otherwise justified and authorized |
| • less than 50 mg | The whole contents of each container |
| • 50 mg or more but less than 300 mg | Half the contents of each container but not less than 50 mg |
| • 300 mg – 5 g | 150 mg |
| • greater than 5 g | 500 mg |
Reference: WHO, Document QAS/11.413 FINAL, March 2012