Standard Operating Procedure for Operation and Cleaning of HPLC Pump (Make: Waters, 515 Pump, Ver 1.0)

Standard Operating Procedure for Operation and Cleaning of HPLC Pump
Standard Operating Procedure for Operation and Cleaning of HPLC Pump

Standard Operating Procedure for Operation and Cleaning of HPLC Pump (Make: Waters, 515 Pump, Ver 1.0)

1. Purpose:

To lay down a procedure for Operation and Cleaning of Operation and Cleaning of High Performance Liquid Chromatography (HPLC) Pump (Make: Waters, 515 Pump, Ver 1.0).

2. Scope:

The scope of this SOP is applicable for Operation and Cleaning of Operation and Cleaning of HPLC Pump (Make: Waters) in Quality Control (QC) laboratory at [company name].

3. Responsibility:

QC Personnel: To operate and clean HPLC Pump.

QC Supervisor: To verify operation and cleaning of HPLC Pump.

QC Head: To ensure compliance to the procedure.

4. Definitions:

HPLC – High Performance Liquid Chromatography

5. Procedure:

5.1 Procedure for Operation of HPLC pump:

• Ensure that all the cables and power cords of the pumps are connected properly.

• Switch “ON” the HPLC Pump. The display will show, 515 Pump, 100 µl, Ver 1.0

• After few seconds, the display will show, Flow 1.000, Ready, 0.000 ml, – 25 psi

• Press “Menu” till purge appears on screen.

• Turn the black valve anticlockwise to purge the mobile phase.

• Press EDIT/ ENTER key. The display will show message ‘Start PURGE’ and ‘Press ENTER to confirm’.

• Press the EDIT/ ENTER key to start purging.

• The system will start countdown from 5 minutes to zero. The display will show, PURGE 5:00, RUN, 5.000 ml, -25 psi.

• After purging close the valve by turning it clockwise.

• Press “Menu” till ‘Flow’ appears on screen.

• Press EDIT/ ENTER Key to change the flow rate. Press the Up “∆” and Down “↓” arrow keys to set the required flow.

• Press “Menu” key to register the set flow.

• Record the operation carried out using the HPLC pump in respective format for recording.

5.2 Procedure for Cleaning of HPLC pump:

• Clean the HPLC pump with dry clean cloth.

• Ensure proper cleaning of HPLC pump.

• Record the cleaning of HPLC pump in respective format for recording.

6. Frequency:

During every operation and cleaning.

7. Formats:

7.1 HPLC pump operation and cleaning record.

Sr. No.

Date

Start time

Activity done

End time

Operation done by

Verified by

Cleaning done by

Cleaning verified by

To refer more similar procedures of quality control laboratory, click here.

To refer more SOP of Pharmaceutical Industry, click here

To read other similar procedure and topics, refer following links:

Standard Operating Procedure of Pharmaceutical Quality Control Laboratory

Standard Operating Procedure for Sampling of Raw Materials in Pharmaceuticals

Standard Operating Procedure for AQL and Sampling of Packaging Materials (PM) in pharmaceuticals

Standard Operating Procedure for Mobile Phase preparation and Handling

Standard Operating Procedure for Receipt, Handling, Qualification and Numbering of HPLC and UPLC Column

Complete guide on High Performance Liquid Chromatography (HPLC)

Interview Questions and Answers on High Performance Liquid Chromatography (HPLC) and Troubleshooting

Dissolution Profile Comparisons, F1 and F2

Acceptance Value for Uniformity of dosage units test as per USP chapter <905> Uniformity of dosage units test

USP NF New and Updated Notices

Articles on Laboratory quality management

Scroll to Top