Standard Operating Procedure for Collection and Disposal of HPLC Waste and UPLC Waste

1. Purpose:

To lay down a procedure for Collection and Disposal of HPLC Waste and UPLC Waste.

2. Scope

The scope of this is applicable for Collection and Disposal of HPLC Waste and UPLC Waste in the pharmaceutical quality control laboratory at [company name].

3. Responsibility

Quality control person: Collection and Disposal of HPLC Waste and UPLC Waste as per laid down procedure

Quality control supervisor: To monitor material operation

Head Quality control: Effective implementation of SOP

4. Definitions

Not Applicable

5. Procedure

5.1 Handling Procedure:

• While HPLC/ UPLC system runs, it generates waste which is the mixture of mobile phase, standard, diluent, and other solvents/ liquid used during the analysis.

• The tubing of waste generated shall be kept in the waste bottle.

• The Waste Bottle shall be placed at lower height from the HPLC system, preferably under the platform of HPLC/ UPLC system.

• Ensure that the waste solvent tube line shall be kept inside the waste bottle and it does not left outside the bottle accidentally.

• Accidental spillage my cause working floor slippery laboratory floor and solvents in the waste may cause contamination of laboratory atmosphere and my impact on sensitive analysis.

• Once the tubing is placed in the waste bottle, bottle opening shall be covered adequately sot that no solvents could evaporate from the bottle.

• Whenever glass bottle is used to collect the waste, it shall be kept in protection container so the accidental fall down of bottle could be prevented or accidental brakeage of the bottle could be prevented.

• Bottle shall be labeled as “HPLC/ UPLC Waste”.

• Always use Amber colored bottle with capacity around 2.5 Liters.

• For each HPLC and HPLC, dedicate one waste bottle.

• The HPLC waste/ UPLC waste should be removed after every analysis to prevent any mix-ups of incompatible solvents or chemicals.

• While doing movement of waste glass containers, always rubber buckets for safe movements.

• Place beaker at each HPLC/ UPLC for collecting waste from pump portion while purging.

• Beaker shall be labeled as “HPLC/ UPLC Waste”.

5.2 Disposal procedure of HPLC waste and UPLC waste:

• Collect the HPLC waste and UPLC waste as and when required and most preferable after each analysis.

• Handle the Waste and Bottle carefully while movement.

• Use appropriate Personnel Protective Equipment (PPE) such as laboratory coat, Nose Mask, Safety Goggles while collecting and disposing the HPLC/ UPLC waste rubber bucket for movement.

• It is analysis responsibility to transfer the waste solvent containers to respective washing area.

• Collect the HPLC/ UPLC waste in a plastic wide mouth buckets and dispose the waste into sink under running tap water.

• Clean the HPLC/ UPLC waste bottle as per glassware washing Standard Operating Procedure.

• After cleaning, place the HPLC/ UPLC waste bottle at the designated place.

6. Frequency

• As per detailed in the Standard Operating Procedure

7. Formats

Not applicable

To read other similar procedure and topics, refer following links:

Standard Operating Procedure of Pharmaceutical Quality Control Laboratory

Standard Operating Procedure for Sampling of Raw Materials in Pharmaceuticals

Standard Operating Procedure for AQL and Sampling of Packaging Materials (PM) in pharmaceuticals